You can create an investment fund as the new commercial enterprise that makes loans or equity investments into a number of job creating entities. Current policies allow for business portfolios within the EB-5 framework. Exiting an EB-5 investment would need to be after the removal of conditions to permanent residency, but many projects have a 5-6 year exit.
The pooling of investors funds can legally occur in a Direct EB-5 and EB-5 Regional Center infrastructure. The best advice is to retain an experienced EB-5 attorney to advise you on how to set up an EB-5 structure to be EB-5 compliant and reach your EB-5 goals. The timing of the commitment of these funds will depend on how and when the jobs will be created and whether each multiple business should be treated as a separate or one EB-5 project.