Question:

Having an application for another visa denied does not disqualify an applicant from receiving EB-5 status. Rejection in the past does not disqualify an applicant unless the reasons are related to immigration fraud or other major problems involving one’s criminal or medical history. All criminal, medical, or U.S. immigration history problems should be disclosed to legal counsel before filing an EB-5 application.

Yes, your previous visa denial does not affect EB-5.

It depends on the reason for the prior denial. An old immigration violation (especially marriage fraud) may make you ineligible for anything ever again. Waivers are available for some past problems and the mere passage of time can cure other problems BUT certain issues ban you from the U.S. for life.